A novel UHPLC-HRMS method for simultaneous determination of 20 amino metabolites and proteins in lymphoma patients' cells and serum.

ABSTRACT: Highly sensitive and selective monitoring of amino metabolites such as glutamine, arginine, tryptophan and related proteins played significant roles in early diagnosis and warning of lymphoma. But those limited abundance and lacked chromophore group in vivo were bottleneck of multivariate analysis. This work aims to develop a novel UHPLC-Triple-TOF-HRMS method for simultaneous quantitation of 20 kinds of amino metabolites and tracing different proteins based on a new mass spectrometry probe (3-bromopropyl) triphenylphosphonium (3-BMP) with ability of enhance ionization efficiency and targeted labeling amino functional groups. An excellent linearity with R² ≥ 0.9995 and inter- and intra-day RSD were 1.43-5.22% and 1.22-5.87%, respectively. Satisfactory recoveries were 87.09-95.82

SUBMITTER: Zhang M 

PROVIDER: S-EPMC12219192 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature