Divergent multifunctional P450s-empowered biosynthesis of bioactive tripterifordin and cryptic atiserenoids in Aconitum implies convergent evolution.

ABSTRACT: Diterpenoids exhibit remarkable structural diversity and bioactivities, shaped primarily by the tandem actions of skeleton-forming terpene synthases (TPSs) and cytochrome P450 monooxygenases. The ent-kaurene and ent-atiserene are labdane-derived diterpene scaffolds for the biosynthesis of diverse bioactive diterpenoids and diterpene alkaloids, including the clinically used analgesic 3-acetylaconitine and anti-arrhythmic guan-fu base A in Aconitum spp., yet what and how P450s drive their structural and functional diversification remain largely unexplored. Here, via mining the transcriptomes of Aconitum carmichaelii and Aconitum coreanum followed by functional validation, we discover nine TPSs capable of furnishing the ent-kaurene, ent-atiserene or 16α-hydroxy-ent-kaurene scaffold and 14 div

SUBMITTER: Luo F 

PROVIDER: S-EPMC12219214 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature