Systematic design and evaluation of artificial CO₂ assimilation pathways.

ABSTRACT: With the advancement of industry and bio-agriculture, the effective management of CO₂ has emerged as a critical challenge for humanity. This study systematically explores multiple CO₂ assimilation pathways using the comb-FBA algorithm, aiming to identify efficient artificial carbon fixation pathways. By extracting 49 CO₂ and HCO₃ ^- involved reactions and combining them with 6,529 reactions from MetaCyc, we constructed the computational set for analysis. These 16 core reactions give rise to 136 carbon fixation pathways for single C₂ targets (such as acetyl-CoA, glyoxylate, and oxalate) and 576 carbon fixation pathways for single C₃ targets (including glyceraldehyde-3-phosphate or pyruvate). Based on these core reac

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12219351 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature