Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids.

ABSTRACT: Understanding SARS-CoV-2 human protein-protein interactions (PPIs) and the host response to infection is essential for developing effective COVID-19 antivirals. However, how the ancestral virus and its variants remodel virus-host protein assemblies in various organ-specific cells and bodily fluids remains unclear. Here, we conduct 639 affinity-purifications by tagging and expressing 28 SARS-CoV-2 and spike proteins from the ancestral virus and four variants in eight cell lines representing five mammalian organs and the immune system. Using mass spectrometry (MS), we identify both known and previously unreported SARS-CoV-2-human PPIs, highlighting similarities and differences across organ- or immune-derived cell lines and virus strains. Besides verifying the cell- and variant-specific PPIs,

SUBMITTER: Broderick K 

PROVIDER: S-EPMC12219648 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature