Comprehensive characterization of human alveolar epithelial cells cultured for 28 days at the air-liquid interface.

ABSTRACT: Lung epithelia are exposed to various substances in the atmosphere through breathing. To investigate the mechanisms and treatments for respiratory diseases caused by these substances, robust in vitro lung epithelial models are essential. This study aimed to develop an in vitro alveolar epithelial model using primary human pulmonary alveolar epithelial cells (HPAEpiCs). HPAEpiCs were cultured at an air-liquid interface (ALI) for 28 days in a medium supplemented with three small molecules: Y-27632, A-83-01, and CHIR99021. The characteristics of the ALI-cultured cells were then analyzed. Immunostaining revealed that many cells expressed alveolar type 2 (AT2) cell markers, such as surfactant protein B and prosurfactant protein C. Single-cell gene expression analysis further confirmed that the

SUBMITTER: Tanabe I 

PROVIDER: S-EPMC12219739 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature