Integrated network pharmacology, transcriptomics, and experimental validation to explore the mechanism of Qihuang Jianpi Zishen granules against Sjogren's syndrome.

ABSTRACT: This study aimed to explore the molecular mechanisms underlying the therapeutic effects of Qihuang JianPi ZiShen granule (QJZG) in treating Sjögren's syndrome (SS) using a combination of network pharmacology, transcriptomics, and experimental validation. We conducted differential expression analysis, weighted gene co-expression network analysis, and utilized GeneCards along with other databases to identify potential therapeutic targets for SS. Concurrently, the active ingredients of QJZG and their associated targets were extracted from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. Network pharmacology was then employed to predict the therapeutic targets and biological pathways through which QJZG may exert its effects on SS. To validate these predicti

SUBMITTER: Wang H 

PROVIDER: S-EPMC12219825 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature