Semaphorin4B is elevated in rheumatoid arthritis and enhances the inflammatory phenotype of macrophages and fibroblast-like synoviocytes.

ABSTRACT:

Background

Several members of the class 4 semaphorins are involved in the pathogenesis of rheumatoid arthritis (RA), regulating proinflammatory functions, but the role of (Sema)phorin4B remains unexplored. Therefore, the aim of this study was to determine the expression and function of Sema4B in RA.

Methods

Peripheral blood monocytes from healthy controls (HC) and patients with RA were differentiated into M1 macrophages and stimulated with Sema4B and LPS alone and in combination. Fibroblast-like synoviocytes (FLS) from patients with osteoarthritis (OA) and RA and 3D synovium micromasses, formed by RA FLS and RA monocytes, were stimulated with Sema4B and TNF-α alone and in combination. PlexinB2 expression was knocked down using siRNA. Synovial mRNA expression was obtained fro

SUBMITTER: Martinez-Ramos S 

PROVIDER: S-EPMC12219977 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature