Construction of a prostate adenocarcinoma molecular classification: integrating spatial transcriptomics with retrospective cohort validation.

ABSTRACT:

Background

Prostate adenocarcinoma (PRAD) is a biologically heterogeneous disease threatening the health of elderly males worldwide, and some PRAD subtypes with certain molecular landscape are always associated with poor prognosis. A more precise molecular classification system for PRAD is urgently needed.

Methods

Through spatial transcriptome analysis, we identified different malignant cell/spot types in PRAD. Then, Monocle 2 analysis was applied to identify malignant cell fates and differentiation-related genes. Together with the prognosis-related genes identified through Kaplan-Meier analysis and univariate Cox regression in TCGA-PRAD cohort, we defined malignant cell differentiation-related prognostic genes (MDPGs). Based on MDPGs, we constructed a malignant cell differe

SUBMITTER: Lu B 

PROVIDER: S-EPMC12220030 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature