Extensive data mining uncovers novel diversity among members of the rare biosphere within the Thermoplasmatota.

ABSTRACT:

Background

Rare species, especially of the marine sedimentary biosphere, have long been overlooked owing to the complexity of sediment microbial communities, their sporadic temporal and patchy spatial abundance, and challenges in cultivating environmental microorganisms. In this study, we combined enrichments, targeted metagenomic sequencing, and extensive data mining to uncover uncultivated members of the archaeal rare biosphere in marine sediments.

Results

In protein-amended enrichments, we detected the ecologically and metabolically uncharacterized class Candidatus Penumbrarchaeia within the phylum Thermoplasmatota. By screening more than 8000 metagenomic runs and 11,479 published genome assemblies, we expanded the phylogeny of Ca. Penumbrarchaeia by 3 novel orders. All s

SUBMITTER: Maeke MD 

PROVIDER: S-EPMC12220078 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature