Genes involved in the regulation of alkaloid and flavonoid biosynthesis in different tissues of Sophora tonkinensis via transcriptomics and metabolomics.

ABSTRACT:

Background

Sophora tonkinensis Gagnep is a significant Chinese herbal medicine, primarily composed of alkaloids and flavonoids, which are its key pharmacological components. Despite its importance, the metabolic pathways of these substances in S. tonkinensis remain inadequately explored.

Results

This study investigates the molecular regulation of alkaloid and flavonoid accumulation in S. tonkinensis. Through high-throughput transcriptome sequencing (RNA-seq) and liquid chromatography-mass spectrometry (LC-MS) of seeds, leaves, stems, and roots, the research identifies differential metabolites and genes involved in alkaloid and flavonoid biosynthesis. The transcriptome analysis reveals 2,727 differentially expressed genes (DEGs), with 35 related to alkaloids and 48 to flavono

SUBMITTER: Liang Y 

PROVIDER: S-EPMC12220083 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature