A dynamic molecular landscape in colorectal cancer progression at single-cell resolution.

ABSTRACT:

Background

Precursor lesions like polyps and adenomas in the colon commonly precede colorectal cancer (CRC), advancing through the "normal-polyp-adenoma-carcinoma" sequence towards malignancy. Yet, the cellular heterogeneity and molecular mechanisms involved in CRC development remain inadequately characterized.

Methods

To understand the molecular mechanisms driving the onset and progression of CRC, we conducted a comprehensive analysis of ten clinical colorectal samples representing sequential pathological stages using single-cell RNA sequencing (scRNA-seq). Validation was performed through immunofluorescence and immunohistochemistry analyses in a separate human colorectal tissue cohort. Additional verification was carried out using bioinformatics analyses of public TCGA and

SUBMITTER: Sheng J 

PROVIDER: S-EPMC12220184 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature