The mechanism of PGR-free direct organogenesis in Lycium ruthenicum leaf explants revealed by RNA-Seq and phytohormone metabolome co-analysis.

ABSTRACT:

Background

Lycium ruthenicum is an economically important shrub known for its resistance to drought and saline-alkali conditions. This study identified a particular L. ruthenicum clone whose leaf explants can undergo direct organogenesis on plant growth regulator (PGR)-free media, and both rooting and shooting abilities of the leaf-tip explants were significantly greater than those of the leaf-middle explants. However, the underlying mechanisms remain unclear.

Results

RNA-Seq analysis revealed that the differentially expressed genes (DEGs) associated with rooting and shooting in both leaf-tip and leaf-middle explants were enriched in the Plant hormone signal transduction KEGG pathway. For the first time, we identified 16 and nine differentially accumulated metabolites (DAMs)

SUBMITTER: Li L 

PROVIDER: S-EPMC12220515 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature