Purification, characterization, and anti-cancer activity of methionine gamma-lyase from a native strain of Pseudomonas mosselii for human cancer treatment.

ABSTRACT:

Aims

Methionine gamma-lyase (MGL) specifically targets L-methionine-dependent cancer cells, making it a promising candidate for anti-cancer drug development. This study aims to purify and characterize L-methioninase from Pseudomonas mosselii and evaluate its potential anti-cancer properties.

Methods and results

MGL was purified through heat treatment, ion exchange chromatography, and gel filtration, achieving a 6-fold purification and a 58.43% recovery rate. The enzyme displayed an activity of 61.16 U/mg and had a molecular weight of 48 kDa. Optimal activity was observed at a pH of 6 and temperatures ranging from 30 to 37℃. Kinetic studies revealed a Km value of 8.458 mM and a Vmax of 0.2702 U/mL/min for L-methionine. The anti-cancer effects of MGL were tested on MCF-7, MOLT

SUBMITTER: Nasirian M 

PROVIDER: S-EPMC12220622 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature