In silico design and evaluation of a multiepitope vaccine against Bordetella pertussis: structural, immunological, and biological properties.

ABSTRACT:

Introduction/objectives

Despite widespread vaccination, the increasing incidence of pertussis underscores the urgent need for innovative vaccine strategies. This study aims to design and analyze, using in silico methods, a multiepitope protein that incorporates epitopes from the S1 subunit of pertussis toxin and the type 1 immunodominant domain of filamentous hemagglutinin (F1). The goal is to enhance both systemic and mucosal immunity through the incorporation of the C-terminal fragment of Clostridium perfringens enterotoxin (C-CPE).

Methods

Using reverse vaccinology, we predicted immunogenic epitopes for lymphocytes derived from the S1 and F1 proteins. The epitopes were assembled into a multiepitope construct named mF1S1-C-CPE, which was then evaluated for its physicochemi

SUBMITTER: Souod N 

PROVIDER: S-EPMC12220642 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature