Chimeric mis-annotations of genes remain pervasive in eukaryotic non-model organisms.

ABSTRACT:

Background

Accurate annotation of protein-coding genes is critical for genome analysis in non-model organisms. However, limited RNA-Seq data and incomplete protein resources can lead to errors, including chimeric gene mis-annotations, where two or more adjacent genes are incorrectly fused into a single model. These errors often persist due to annotation inertia, where mistakes are propagated and amplified through data sharing and reanalysis, and leads to cases where the mis-annotated model becomes favoured over the correct model. This complicates almost all downstream genomic analyses such as gene expression studies and comparative genomics.

Results

We investigated chimeric mis-annotations across 30 recently annotated genomes spanning invertebrates, vertebrates, and plants,

SUBMITTER: Bachler A 

PROVIDER: S-EPMC12220653 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature