The Drosophila histone variant H2Av facilitates Notch signaling activity in a two-tier regulatory fashion.

ABSTRACT:

Background

H2Av is an evolutionarily conserved H2A variant protein involved in the regulation of transcription. The Tip60 complex is recruited by different transcription factors to facilitate the incorporation and acetylation of H2Av, thereby influencing target gene expression. The Tip60-H2Av axis is involved in various developmental processes, though its precise roles are not yet fully understood.

Methods

RNA interference and gene mutation technology were used to screen essential genes in regulating Notch signaling pathway. Immunostaining was used to detect the protein level of H2Av, Tip60 complex as well as Notch signaling pathway components. Chromatin immunoprecipitation assays were performed to detect the specific binding site of H2Av in E(spl)-Complex and Su(H) genes.

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12220669 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature