A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effector.

ABSTRACT:

Background

Optogenetic systems use light-responsive proteins to control gene expression, ion channels, protein localization, and signaling with the "flip of a switch". One such tool is the light activated CRISPR effector (LACE) system. Its ability to regulate gene expression in a tunable, reversible, and spatially resolved manner makes it attractive for many applications. However, LACE relies on delivery of four separate components on individual plasmids, which can limit its use. Here, we optimize LACE to reduce the number of plasmids needed to deliver all four components.

Results

The two-plasmid LACE (2pLACE) system combines the four components of the original LACE system into two plasmids. Following construction, the behavior of 2pLACE was rigorously tested using optogenet

SUBMITTER: Garimella SS 

PROVIDER: S-EPMC12220775 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature