Contribution of perineuronal nets to hyperexcitability in pilocarpine-induced status epilepticus.

ABSTRACT:

Objective

Changes in extracellular matrix (ECM) and highly condensed ECM structures called perineuronal nets (PNNs) have been reported in human patients with epilepsy as well as some animal models of epilepsy. We studied potential ECM changes in a mouse model of pilocarpine-induced status epilepticus (PISE) and their potential contributions to seizures.

Methods

We used a reduced intensity pilocarpine model to induce status epilepticus (SE) in mice. Wisteria floribunda agglutinin (WFA) staining was used to identify PNNs. Immunohistochemistry, patch-clamp electrophysiology, drug studies, and transcriptomic analysis were used to assess changes in gene expression and physiological properties of the PNN-surrounded cells.

Results

WFA immunohistochemical staining shows de no

SUBMITTER: Woo AM 

PROVIDER: S-EPMC12221199 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature