In vivo pulse-chase in Caenorhabditis elegans reveals intestinal histone turnover changes upon starvation.

ABSTRACT: The ability to study protein dynamics and function in the authentic context of a multicellular organism is paramount to better understand biological phenomena in animal health and disease. Pulse-chase of self-labeling fusion protein tags provides the opportunity to label proteins of interest and track those proteins over time. There are currently several challenges associated with performing in vivo protein pulse-chase in animals, such as cost, reproducibility, and accurate detection methods. The Caenorhabditis elegans model organism has attributes that alleviate many of these challenges. This work tests the feasibility of applying the Halo modified enzyme (HaloTag) for in vivo protein pulse-chase in C. elegans. HaloTag intestinal histone reporters were created in the worm and used to demo

SUBMITTER: Borchers C 

PROVIDER: S-EPMC12221288 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature