MicroRNA-129-5p-mediated translational repression of microglial ROCK1 leads to enhanced phagocytosis.

ABSTRACT: ROCK1 plays an important role in phagocytosis by inducing cytoskeletal rearrangement. Although the transcriptional regulation of ROCK1 is known but its post-transcriptional regulation is underexplored. We intended to find a mechanism of microglial phagocytosis through possible post-transcriptional regulation of ROCK1. The study identified miR-129-5p as a regulator of microglial phagocytosis following exposure to an environmental stressor, arsenic, combining in silico analysis, mutational analysis, in vitro experiments, and validation in BALB/c mouse. The in silico analysis and in vitro studies with mouse primary neonatal microglia, BV2 microglia, ex vivo microglia, and human microglial cell line CHME3 revealed that arsenic exposure increases microglial phagocytosis. Arsenic exposure was al

SUBMITTER: Dey RK 

PROVIDER: S-EPMC12221357 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature