In silico analysis of hypothetical proteins in Pseudomonas aeruginosa PAC1: Structural and functional insights.

ABSTRACT:

Background

Bacterial genomes contain numerous hypothetical proteins (HPs) with uncharacterized roles. This study used computational methods to identify and predict the functions of such proteins in the Pseudomonas aeruginosa PAC1 strain.

Methods

The PAC1 genome (GenBank: CP053706.1) was analyzed, starting with 828 HPs. Proteins shorter than 50 amino acids (unlikely to form stable structures) were excluded, leaving 807 HPs. Physicochemical properties were assessed to filter unstable proteins, resulting in 272 candidates. Subcellular localization tools predicted cytoplasmic localization for 58 proteins. Functional annotation identified conserved domains, and homology modeling generated 3D structures for proteins with >80 % similarity to known templates. Structural validation a

SUBMITTER: Abdallah MM 

PROVIDER: S-EPMC12221643 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature