EndoMAP.v1 charts the structural landscape of human early endosome complexes.

ABSTRACT: Early or sorting endosomes are dynamic organelles that play key roles in proteome control by triaging plasma membrane proteins for either recycling or degradation in the lysosome^1,2. These events are coordinated by numerous transiently associated regulatory complexes and integral membrane components that contribute to organelle identity during endosome maturation³. Although a subset of the several hundred protein components and cargoes known to associate with endosomes have been studied at the biochemical and/or structural level, interaction partners and higher-order molecular assemblies for many endosomal components remain unknown. Here, we combine crosslinking and native gel mass spectrometry^4-7 of purified early endosomes with AlphaFold^8,9 and

SUBMITTER: Gonzalez-Lozano MA 

PROVIDER: S-EPMC12222028 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature