Continuous nuclear envelope surveillance is required for DNA double strand break repair.

ABSTRACT: Precise double-strand break (DSB) repair is paramount for genome stability. Homologous recombination (HR) is preferred to repair DSBs when a nearby sister chromatid ensures an error-free template. In Saccharomyces cerevisiae, this preference extends into anaphase and telophase (late mitosis; late-M) despite sister chromatids having been pulled apart. Previously, we identified the nuclear envelope (NE) protein Msc1 as important for late-M DSB repair. Here, we report that Msc1 faces the NE lumen, and its depletion leads to DSB-independent over-compartmentalization of the nucleus and nuclear pore complex (NPC) mislocation. These phenotypes are partly shared by the highly conserved NE healing complex ESCRT-III. Finally, we show that these abnormal NE phenotypes are found throughout the cell cy

SUBMITTER: Medina-Suarez S 

PROVIDER: S-EPMC12222759 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature