Engineered phages for selective adsorption of rare earth elements.

ABSTRACT: Biomaterial extraction of rare earth elements (REEs) offers an environmentally friendly and efficient alternative to chemical solvent methods. M13 Phages showing less non-specific REE binding without glycosylated polysaccharides have multiple copies of p8 fusions to display peptides with selective binding for the desired REEs. In this study, we developed two diverse phage display libraries: one displaying 12-mer peptides and another displaying 17-mer circular peptides, each with over 1 × 107 unique peptides. Six fluorescent protein-peptide fusions, CFP-peptide-YFP, were prepared for measuring Kd. Their REE affinity, ranging from 2 to 30 micromolar (µM), were generally weak, but a novel peptide-phage, H11G-p8, a derivative of H11-p8, showed a better affinity toward Eu than others. Recombina

SUBMITTER: Ditzel AM 

PROVIDER: S-EPMC12223077 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature