Volatile dimethyl disulphide emission from Burkholderia cepacia LS-044 suppresses metabolism and budding in caspofungin-resistant Nakaseomyces glabratus NT2.

ABSTRACT: Some bacteria emit dimethyl disulphide (DMDS), one of the bioactive volatile sulphurous compounds (VSCs) of environmental and ecological significance, yet unexplored in combating drug-resistant yeasts. Here, we show the anti-budding and fungicidal activity of volatile DMDS emitted from Burkholderia cepacia LS-044 on the caspofungin-resistant yeast Nakaseomyces glabratus NT2. We identified a gene encoding L-methionine-γ-lyase (mdeA) catalysing DMDS formation in LS-044 and detected volatile DMDS as one of the VSCs (12% peak area) emitted by LS-044 through solid-phase microextraction followed by gas chromatographic-mass spectrometry. Exposure to volatiles of LS-044 resulted in a significant decline in metabolism (~ 98%), media alkalinity (~ 26%), and viable cell count (52‒95%) of NT2. Confoca

SUBMITTER: Rahmath NAK 

PROVIDER: S-EPMC12223190 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature