Design of a Universal Backbone Improving the Endonuclease Dependence Molecular Beacon Assay.

ABSTRACT: The endonuclease dependence molecular beacon assay (DEMBA) is an isothermal detection method celebrated for its high sensitivity, operational ease, and time-saving capabilities. However, the intricate design of the probes limits the extensive application of DEMBA. The main challenge is the requirement for the probe sequence to include restriction endonuclease sites, two target sequence binding sites, and self-binding sites, which frequently overlap. To overcome this issue, we introduce a strategy involving a universal probe backbone. This design permits the modification of only the target sequence binding sites for the detection of various nucleic acids. In this research, we successfully created universal backbone probes and implemented them in DEMBA assays. Our results demonstrate that th

SUBMITTER: Liu Z 

PROVIDER: S-EPMC12223834 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature