EGFRp4 Peptides: A Novel Strategy for Epidermal Growth Factor Receptor (EGFR) Inhibitor.

ABSTRACT: The epidermal growth factor receptor (EGFR) plays a pivotal role in cell signaling pathways, and its malfunction is implicated in cancers such as lung, breast, and gastric cancer. Numerous studies have explored EGFR-specific peptides using various methods, excluding ribosome display peptide libraries. In this study, EGFRp1 and EGFRp4 peptides (11-mer) were discovered through ribosome display selection against the extracellular domain of EGFR (EGFR ECD or sEGFR). Notably, EGFRp4 exhibited superior binding affinity within the nanomolar range compared with other reported EGFR-specific peptides. It also demonstrated a positive cooperativity model with EGFR ECD across three experimental approaches: fluorescence polarization, surface plasmon resonance, and ligand tracer. Molecular docking substa

SUBMITTER: Jiwacharoenchai N 

PROVIDER: S-EPMC12223836 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature