Hydrophilic Interaction Chromatography HRMS with Acrylamide Monolithic Columns: A Novel Approach for Intact Antibody Glycoform Characterization.

ABSTRACT: Glycosylation significantly impacts the pharmacokinetics and efficacy of monoclonal antibody (mAb) biotherapeutics. Characterizing mAbs' glycoform profiles is crucial for optimizing therapeutic outcomes, and intact antibody analysis provides key information about the glycoform combinations present. While state-of-the-art RPLC-MS methods are commonly used for intact mAb analysis, they lack the selectivity to resolve glycoforms and, therefore, may not detect lower-abundance glycoforms. In contrast, HILIC methods have demonstrated good resolving power for middle-up mAb glycoform analysis. However, to date, no application of HILIC has been described to characterize mAb glycoforms at the intact level. This study describes the development of acrylamide monoliths for HILIC-MS intact mAb glycoprof

SUBMITTER: van der Zon AAM 

PROVIDER: S-EPMC12224157 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature