Exploring the Impact of Biotic and Abiotic Surfaces on Protein Binding Modulation and Bacteria Attachment: Integrating Biological and Mathematical Approaches.

ABSTRACT: The oral environment is composed of a diverse array of proteins, and any substrate inserted into this habitat promptly becomes subjected to protein adsorption and bacterial colonization. However, the predictive and modulatory nature of implant surfaces coated with salivary pellicle proteomes in microbial adhesion has not been explored using high-throughput techniques. Thus, using human saliva for salivary pellicle adsorption and microbial accumulation, we compared adsorption and community formation on titanium (Ti) biomaterials (implant devices) and dental surfaces (enamel and dentine). The proteomic profile was evaluated by liquid chromatography coupled with tandem mass spectrometry, and the microbiome was assessed using 16S RNA sequencing. Linear discriminant analysis (LDA) and canonical

SUBMITTER: Souza JGS 

PROVIDER: S-EPMC12224314 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature