Spatial phenotyping of human bronchial airways in obstructive lung disease.

ABSTRACT: Chronic respiratory diseases such as asthma and COPD involve interactions between multiple resident and immune cell types within bronchial airways, resulting in structural and functional changes. Thus cellular heterogeneity, arrangements and associated neighborhoods as well as interactions between cells and matrices represent intriguing yet challenging areas of study. Spatial phenotypic profiling facilitates exploration of these issues of the cellular microenvironment and identification of context-dependent cell-cell interactions. Utilizing spatial phenotyping, we interrogated the features and cellular landscape of lungs from non-asthmatics, asthmatics, and COPD in FFPE samples by developing a 10-plex antibody panel for the Akoya PhenoCycler®-Fusion system, focused on immune cells (CD45, C

SUBMITTER: Khalfaoui L 

PROVIDER: S-EPMC12224523 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature