A dual-drug strategy to enhance the function of cryopreserved ovaries by promoting revascularization and inhibiting follicle over-activation.

ABSTRACT:

Background

Ovarian tissue cryopreservation and transplantation (OTCT) is a promising approach for fertility preservation. However, significant follicle loss after transplantation challenges long-term reproductive recovery. Although primordial follicle loss and ischemic damage are known contributors, the underlying mechanisms and effective strategies to mitigate these damages are still lacking.

Methods

Ovarian tissues from wild-type or Tek-CreER^T2;mTmG female mice were cryopreserved using vitrification. Follicle dynamics after OTCT were analyzed through histology, proteomics, and high-resolution imaging. To assess protective effects, cryopreserved ovaries were treated with 500 nM rapamycin (Rapa), an inhibitor of primordial follicle activation; 5 ng/mL vascular end

SUBMITTER: Li L 

PROVIDER: S-EPMC12224590 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature