Facilitating crRNA Design by Integrating DNA Interaction Features of CRISPR-Cas12a System.

ABSTRACT: The CRISPR-Cas12a system has gained significant attention as a rapid nucleic acid diagnostic tool due to its crRNA-guided trans-cleavage activity. Accurately predicting the activity of different targets is significant to facilitate the crRNA availability but remains challenging. In this study, a novel approach is presented that combines molecular dynamics simulations and neural network modeling to predict the trans-cleavage activity. Unlike conventional tools that rely solely on the base sequences, our method integrated sequence features and molecular interaction features of DNA in the CRISPR-Cas12a system, significantly improving prediction accuracy. Through feature importance analysis, key sequence features that influence Cas12a trans-cleavage activity are identified. Additionally, a crR

SUBMITTER: Yao Z 

PROVIDER: S-EPMC12224974 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature