Retinal Explant Culture from Mouse, Human, and Nonhuman Primates and Its Applications in Vision Research.

ABSTRACT: The retinal explant culture system is a valuable tool for studying the pharmacological, toxicological, and developmental aspects of the retina. It is also used for translational studies such as gene therapy. While no photoreceptor-like cell lines are available for in vitro studies of photoreceptor cell biology, the retinal explant culture maintains the laminated retinal structure ex vivo for as long as a month. Human and nonhuman primate (NHP) postmortem retinal explants cut into small pieces offer the possibility of testing multiple conditions for safety and adeno-associated viral (AAV) vector optimization. In addition, the cone-enriched foveal area can be studied using the retinal explants. Here, we present a detailed working protocol for retinal explant isolation and culture from mouse,

SUBMITTER: Vats A 

PROVIDER: S-EPMC12225654 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature