ADAM8 deficiency in macrophages promotes cardiac repair after myocardial infarction via ANXA2-mTOR-autophagy pathway.

ABSTRACT:

Introduction

A disintegrin and metalloproteinase 8 (ADAM8), a crucial regulator in macrophages, is closely associated with cardiovascular disease progression.

Objectives

This study aimed to explore how ADAM8 regulates macrophage function to inhibit cardiac repair after myocardial infarction (MI).

Methods

Macrophage-specific ADAM8 knockout mice (ADAM8^{flox/flox, Lyz2-Cre}, KO) and corresponding control mice (ADAM8^flox/flox, Flox) were established using the CRISPR/Cas9 system. Bone marrow transplantation was performed, and macrophage-specific ADAM8-overexpressing adeno-associated virus (AAV6-CD68-Adam8) was produced. Finally, proteomics, RNA sequencing, and co-immunoprecipitation/mass spectrometry (COIP/MS) were used to explore the underlying mechani

SUBMITTER: Ji Z 

PROVIDER: S-EPMC12225930 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature