GmBSK1-GmGSK1-GmBES1.5 regulatory module controls heat tolerance in soybean.

ABSTRACT:

Introduction

Heat stress poses a severe threat to the growth and production of soybean (Glycine max). Brassinosteroids (BRs) actively participate in plant responses to abiotic stresses, however, the role of BR signaling pathway genes in response to heat stress in soybean remains poorly understood.

Objectives

In this study, we investigate the regulatory mechanisms of GmBSK1 and GmBES1.5 in response to heat stress and the physiological characteristics and yield performance under heat stress conditions.

Methods

Transgenic technology and CRISPR/Cas9 technology were used to generated GmBSK1-OE, GmBES1.5-OE and gmbsk1 transgenic soybean plants, and transcriptome analysis, LUC activity assay and EMSA assay were carried out to elucidate the potential molecular mechanism under

SUBMITTER: Hou ZH 

PROVIDER: S-EPMC12225950 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature