FliA regulates the antibacterial activity of plantaricin BM-1 against <i>Escherichia coli</i> K-12 through the LuxS/AI-2 quorum-sensing-mediated biofilm formation.

ABSTRACT:

Introduction

Plantaricin BM-1 is a class IIa bacteriocin active against Escherichia coli. However, the mode of action of class IIa bacteriocins against gram-negative bacteria remains unclear. In this study, the regulatory role of sigma factor FliA (σ²⁸) in the antibacterial mechanism of plantaricin BM-1 against E. coli K-12 BW25113 is evaluated.

Methods

The fliA-complemented strain of E. coli JW1907, namely E. coli ReJW1907, was constructed through λ-Red homologous recombination. The effects of plantaricin BM-1 on E. coli growth, cell morphology, and membrane integrity were investigated using growth curves, electron microscopy, and flow cytometry. The biofilm formation ability of E. coli was evaluated using crystal

SUBMITTER: Wang S 

PROVIDER: S-EPMC12226578 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature