LINC00662 regulates osteogenic differentiation of BMSCs and inhibits fracture healing via miR-330-3p/PTEN axis.

ABSTRACT:

Background

Surgical treatment alone is not effective in addressing delayed fracture healing (DFH). This study nvestigates the molecular mechanism underlying fracture healing to identify improved therapeutic strategies.

Methods

Serum samples were collected from 76 normal fracture healing (NFH) and 70 DFH patients. RT-qPCR was performed to determine LINC00662, miR-330-3p, and PTEN mRNA expression in serum and Human Bone marrow mesenchymal stem cells (HBMSCs). The diagnostic potential of LINC00662 and miR-330-3p for DFH patients was evaluated via ROC analysis. Binding sites were predicted using bioinformatic databases and validated by dual-luciferase reporter assays. HBMSCs proliferation, apoptosis and osteogenic differentiation were analyzed using CCK-8, flow cytometry, andost

SUBMITTER: Gong J 

PROVIDER: S-EPMC12226875 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature