Metabarcoding Study of Fecal Bacterial and Eukaryotic Pathogens in Stray and Pet Cats in Seoul, South Korea, 2022.

ABSTRACT:

Objectives

Zoonotic diseases pose substantial public health risks because of their potential transmission from animals to humans. As pets, cats can harbor these pathogens. The objective of this study was to describe bacterial and eukaryotic pathogens in the feces of stray and pet cats in Seoul, South Korea, using next-generation sequencing techniques.

Methods

We collected 26 fresh fecal samples (17 from pet cats and 9 from stray cats) in Seoul's Mapo-gu District in April and May 2022. Amplicon sequencing targeted the V4 region of the 16S rRNA gene for bacterial pathogens and the V9 region of the 18S rRNA gene for eukaryotic pathogens. We used QIIME 2 to conduct bioinformatic analysis, assessing alpha diversity with the Shannon Diversity Index and beta diversity with principa

SUBMITTER: Ayala MGO 

PROVIDER: S-EPMC12227441 | biostudies-literature | 2025 Jul-Aug

REPOSITORIES: biostudies-literature