Discovering novel type I collagen fragments from Cyprinus carpio supporting bone regeneration.

ABSTRACT: Fish collagen is gaining increasing attention in tissue engineering due to its exceptional bioactivity. This study aimed to isolate functional fish collagen fragments capable of microbial biosynthesis and supporting bone tissue regeneration. Collagen fragments of 150 amino acids were extracted from Cyprinus carpio collagen I (CcCOL1), and their bioactivity, net charge, and hydrophobicity were calculated and analyzed for correlations, these physicochemical and sequential features were using to train the machine learning model, which classified the fragments into three subgroups. Representative samples were selected from each cluster or directly from the original CcCOL1. Six out of eight variants were successfully secreted in Pichia pastoris, and all formed triple-helical structures, while o

SUBMITTER: Zeng J 

PROVIDER: S-EPMC12227466 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature