Re-evaluation of the <i>C</i>-Glucosyltransferase IroB Illuminates Its Ability to <i>C</i>-Glucosylate Non-native Triscatecholate Enterobactin Mimics.

ABSTRACT: The pathogen-associated C-glucosyltransferase IroB is involved in the biosynthesis of salmochelins, C-glucosylated derivatives of enterobactin (Ent), which is a triscatecholate siderophore of enteric bacteria including Salmonella enterica and Escherichia coli. Here, we reassess the ability of IroB to C-glucosylate non-native triscatecholate mimics of Ent, which may have utility in the design and development of siderophore-based therapeutics and diagnostics. We establish TRENCAM (TC) and MECAM (MC), synthetic Ent analogs with tris(2-aminoethyl)amine- or mesitylene-derived backbones replacing the trilactone core of Ent, respectively, and their monoglucosylated congeners as substrates of IroB. Time course analyses and steady-state kinetic studies, which were

SUBMITTER: Motz RN 

PROVIDER: S-EPMC12228543 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature