Ez-CAZy a reference annotation database for linking glycoside hydrolase sequence to enzymatic activity.

ABSTRACT: Glycoside Hydrolases (GH) are carbohydrate-active enzymes that play a critical role in the degradation of carbohydrates, impacting ecosystem function, human health, and biotechnological applications. The functional annotation of GHs within the CAZy database is hindered by the lack of sequence-specific definitions, annotation tools, and reliance on generalized "majority rule" activity assumptions. Here, we introduce ez-CAZy, a custom reference database designed to link GH sequences, as well as other CAZy, to their enzymatic activities. By reannotating over 7,000 biochemically characterized GHs using Hidden Markov Model profiles and other publicly accessible tools, we provide detailed sequence metadata, domain architectures, and functional predictions. Our analysis reveals the clustered dist

SUBMITTER: Erdody DS 

PROVIDER: S-EPMC12229491 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature