A sensitive sample preparation pipeline for adventitious virus detection using Oxford Nanopore sequencing.

ABSTRACT: Recent regulatory guidance now encourages the use of sequencing as an alternative adventitious agent testing assay to lengthy compendial in vivo assays used for cell line qualification. Most short-read sequencing assays, however, still require over a week to obtain a final test result since the sequencing must be completed before bioinformatic analysis can begin, which is still too long for some cell and gene therapy products that must be released as soon as possible to reach critically ill patients. Oxford Nanopore sequencing can address these issues, as it provides real-time basecalling and sequence alignment, which can reduce the overall assay time. Still, as with any sequencing platform, the abundance of background nucleic acid from the human or mammalian host can mask the signa

SUBMITTER: Tsinda EK 

PROVIDER: S-EPMC12229724 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature