CircEPDR1 regulates proliferation and differentiation of goat skeletal muscle satellite cells through miR-345-3p/Akirin1 axis.

ABSTRACT:

Objective

Circular RNAs (circRNAs) play pivotal roles in regulating the growth and development of mammalian skeletal muscle This study aims to provide new insights into the molecu-lar regulatory network that underlies skeletal muscle development, presenting valuable targets to enhance and optimize livestock production performance.

Methods

In this study, we anchored a novel circRNA from previous circRNA sequencing data from goat skeletal muscle and named it circEPDR1, which was confirmed through en-zymatic digestion and Sanger sequencing. Functional investigation of circEPDR1 included overexpression experiments in goat muscle satellite cells (MuSCs), and its subcellular localization was determined using nuclear-cytoplasmic separation. Bioinformatics analysis was employed to r

SUBMITTER: Shi S 

PROVIDER: S-EPMC12229913 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature