Nanopore direct RNA sequencing of human transcriptomes reveals the complexity of mRNA modifications and crosstalk between regulatory features.

ABSTRACT: The identification and functional characterization of chemical modifications on an mRNA molecule, in particular N⁶-methyladenosine (m⁶A) modification, significantly broadened our understanding of RNA function and regulation. While interactions between RNA modifications and other RNA features have been proposed, direct evidence showing correlation is limited. Here, using Oxford Nanopore long-read direct RNA sequencing (dRNA-seq), we simultaneously interrogate the transcriptome and epitranscriptome of a human leukemia cell line to investigate the correlation between m⁶A modifications, mRNA abundance, mRNA stability, polyadenylation (poly(A)) tail length, and alternative splicing. High-quality dRNA-seq is important for unbiased and large-scale correlative anal

SUBMITTER: Kim Y 

PROVIDER: S-EPMC12230237 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature