A 96-Well Ultrafiltration Approach for the High-Throughput Proteome Analysis of Extracellular Vesicles Isolated From Conditioned Medium.

ABSTRACT: Extracellular vesicles (EVs), nanoscale vesicles that are secreted by cells, are critical mediators of intercellular communication and play a crucial role in diverse pathologies such as cancer development. Therefore, EVs are regarded as having high potential in the clinic, both for diagnostic and therapeutic applications. Unfortunately, EVs reside in complex biofluids and their consistent preparation at sufficient purity for mass spectrometry-based proteomics has proven to be challenging, especially when increased high-throughput is required. Here, we describe the incorporation of our previously reported filter-aided EV enrichment (FAEVEr) strategy for the separation of EVs from conditioned medium, from harvest to proteomic analysis completely to a streamlined 96-well format. We compared o

SUBMITTER: Pauwels J 

PROVIDER: S-EPMC12230347 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature