Multivalent 2D- and 3D-nanogels as carbohydrate-lectin binders.

ABSTRACT: The development of synthetic glycoarchitectures for targeted bacterial adhesion represents a promising strategy in anti-adhesion therapy. This study presents the synthesis and characterization of two distinct mannosylated nanogel architectures. First, a spherical 3D-nanogel was prepared via nanoprecipitation and functionalized with α-D-mannose units. This system demonstrated enhanced precipitation kinetics in turbidity measurements with Concanavalin A and exhibited single-site binding behavior comparable to monovalent reference compounds when tested with intact E. coli strain ORN 178 (FimH⁺) via microscale thermophoresis. Cryo-TEM imaging revealed clear co-localization with bacterial pili, confirming specific bacterial interactions. The complementary sheet-l

SUBMITTER: Schmitt AC 

PROVIDER: S-EPMC12230938 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature