High abundance of antimicrobial resistance genes in chicken flocks receiving antimicrobial treatment in Vietnamese poultry production.

ABSTRACT:

Background and objectives

Studies focussing on measuring antimicrobial resistance (AMR) rely on phenotyping or low throughput PCR detection of limited AMR genes (ARGs); high-throughput qPCR (HT-qPCR) may be a scalable approach for measuring AMR. We applied Fluidigm HT-qPCR to measure the impact of flock-level antimicrobial use (AMU) on genotypic AMR in the Mekong Delta area of Vietnam.

Methods

AMU-related data and pooled faecal samples were collected longitudinally from 20 meat chicken flocks, divided into flocks treated with antimicrobials and untreated controls. Samples were analysed for 94 ARGs using Fluidigm HT-qPCR. Normalized ARG abundance was measured in reference to 16S rRNA. A regression model was constructed to weigh the effect of AMU factors on AMR.

Results

SUBMITTER: Dutta A 

PROVIDER: S-EPMC12231541 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature