Treatment of rheumatoid arthritis by Rhododendron molle G. Don: the anti-inflammatory mechanism of its extract was elucidated by network analysis combined with experimental verification.

ABSTRACT:

Objective

We investigated the underlying mechanism by which Rhododendron molle G. Don extraction (RME) mitigates rheumatoid arthritis (RA) in rats through network analysis and molecular docking.

Methods

The active metabolites of RME were analyzed by high performance liquid chromatography - evaporative light scattering detector (HPLC-ELSD). Subsequently, the potential targets were screened from various databases. Genes relative to RA and GSE55457 data sets were obtained. Furthermore, the biological processes associated with the drug targets and GSE55457 genes were analyzed. A protein - protein interaction (PPI) network was constructed and subjected to topological analysis. Key proteins within this network were identified and validated by in vivo experiments.

Results

HP

SUBMITTER: Mei W 

PROVIDER: S-EPMC12232070 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature