Genomic insights into tigecycline non-susceptibility in Clostridioides difficile: the role of the Tet P determinant and efflux mechanisms.

ABSTRACT:

Background

Clostridioides difficile infection (CDI) is a significant cause of nosocomial infections, often arising from antibiotic-induced disruption of the normal gut microbiota, enabling C. difficile proliferation. Tigecycline has proven effective in treating refractory CDI, with resistance to this antibiotic being rare. However, our previous study identified six C. difficile isolates that were non-susceptible to tigecyclin.

Methods

To investigate the mechanisms underlying reduced tigecycline susceptibility, the genome of C. difficile strain 16338, which is non-susceptible to tigecycline, was characterized. The tetA(P), tetB(P), and Tet P (including 2,000 bp upstream), were individually cloned into recombinant plasmids and transferred into Escherichia coli to assess their

SUBMITTER: Dang Z 

PROVIDER: S-EPMC12232799 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature