Chikungunya masquerading as dengue infection in Sri Lanka uncovered by metagenomics.

ABSTRACT:

Introduction

Dengue is a significant threat to human health in South and Southeast Asia where patients are treated without diagnostic confirmation during outbreaks. This approach, though cost-effective may miss important infections especially those caused by other arboviruses (e.g., Zika, Chikungunya and West Nile virus). This study aimed to diagnose missed infections mimicking dengue by using metagenomic next generation sequencing (mNGS).

Methods and principal findings

Total nucleic acid (DNA and RNA) was extracted and subjected to mNGS from acute infection plasma of 60 patients from a prospective cohort study in Sri Lanka in which patients with clinically suspected dengue fever were recruited but were later confirmed as dengue-negative by NS1 antigen testing and by dengue-

SUBMITTER: Kabir KMA 

PROVIDER: S-EPMC12233278 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature